Figure 9.

Response of cannabis genotypes to callus development on MM without activated charcoal (MM-AC). (A) Callus diameter of four genotypes developing from 1 cm² leaf explants. (B) Callus area from leaf explants of 8 cannabis genotypes compared with the mean callus area across all strains (represented by the dotted line). The “*”represents significance level and “ns” represents not significant relationships between each genotype and across all genotype means (dotted line). A Kruskal–Wallis test resulting in a p of 8 × 10⁻¹¹ and a Games–Howell post-hoc test were performed. Significant differences identified in the Games–Howell post-hoc test are shown using letters above the boxplots of each strain. (C) Growth of Cheesequake (CHQ) callus from leaf and petiole explants after 1 month under 24-h dark and 24-h light conditions on MM. Statistical analysis was performed by Tukey's HSD test with significance at p < 0.05. Bars followed by different letters indicate significant differences. (D) Growth of callus from leaves and petioles of genotype Girl Scout Cookie (GSC) (left) and Space Queen (SPQ) (right). Measurements of diameter were made after 4 weeks. Statistical analysis was performed by Tukey's HSD test with significance at p < 0.05. Bars followed by different letters indicate significant differences.