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. Author manuscript; available in PMC: 2021 Oct 5.
Published in final edited form as: Stem Cells. 2019 May 13;37(7):899–909. doi: 10.1002/stem.3018

Figure 3.

Figure 3.

Heparan sulfate lowers Sonic Hedgehog (Shh) signaling in Ext1/2 null neuralized embryoid bodies (nEBs). (A–F, H–M): Mosaic nEBs of the indicated genotypes, including 2% Shh-expressing cells. Shh−/−;Ptch1+/LacZ cells (A, B, H, I) or Shh−/−;Ptch1+/LacZ;Ext1−/− cells (C, D, J, K) or Shh−/−;Ptch1+/LacZ;Ext2−/− cells (E, F, L, M) and 2% Shh-expressing cells were cultured in control medium (A, C, E, H, J, L) or in medium supplemented with heparan sulfate (B, D, F, I, K, M). (G, N): Quantification of the Shh-mediated induction of Olig2 (G) and Isl1/2 (N) per nEB. One-way analysis of variance with post hoc Dunnett’s test; *, p < .05;***, p < .001; ****, p < .0001. Scale bar is 100 μm.