Table 1.
Gene-specific forward and reverse primer sequences of associated genes
| Gene | Primer sequences |
|---|---|
| NF-κβ | Forward 5’- CCCACACTATGGATTTCCTACTTATGG-3’ |
| Reverse 5’- CCAGCAGCATCTTCACGTCTC-3’ | |
| TNF-α | Forward 5’- CCCAGGCAGTCAGATCATCTTC-3’ |
| Reverse 5’- AGCTGCCCCTCAGCTTGA-3’ | |
| IL6 | Forward 5’- GGTACATCCTCGACGGCATCT-3’ |
| Reverse 5’- GAGGATACCACTCCCAACAGACC-3’ | |
| COX-2 | Forward 5’- GGAGAGACTATCAAGATAGT-3’ |
| Reverse 5’- ATGGTCAGTAGACTTTTACA-3’ | |
| iNOS | Forward 5’- AATGGCAACATCAGGTCGGCCATCACT-3’ |
| Reverse 5’- CTGTGTGTGTCACAGAAGTCTCGAACTC-3’ | |
| GAPDH | Forward 5’- GAAGGTGAAGGTCGGAGTC-3’ |
| Reverse 5’- GAAGATGGTGATGGGATTTC-3’ |
NF-κβ: Nuclear factor kappa beta, TNF-α: Tumor necrosis factor-alpha, IL-6: Interlukin-6, COX: Cyclooxygenase-2, iNOS: Inducible nitric oxide synthase, GAPDH: Glyceraldehyde-3-phosphate dehydrogenase