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. 2021 Aug 31;25(2):279–285. doi: 10.4103/0973-029X.325127

Table 1.

Gene-specific forward and reverse primer sequences of associated genes

Gene Primer sequences
NF-κβ Forward 5’- CCCACACTATGGATTTCCTACTTATGG-3’
Reverse 5’- CCAGCAGCATCTTCACGTCTC-3’
TNF-α Forward 5’- CCCAGGCAGTCAGATCATCTTC-3’
Reverse 5’- AGCTGCCCCTCAGCTTGA-3’
IL6 Forward 5’- GGTACATCCTCGACGGCATCT-3’
Reverse 5’- GAGGATACCACTCCCAACAGACC-3’
COX-2 Forward 5’- GGAGAGACTATCAAGATAGT-3’
Reverse 5’- ATGGTCAGTAGACTTTTACA-3’
iNOS Forward 5’- AATGGCAACATCAGGTCGGCCATCACT-3’
Reverse 5’- CTGTGTGTGTCACAGAAGTCTCGAACTC-3’
GAPDH Forward 5’- GAAGGTGAAGGTCGGAGTC-3’
Reverse 5’- GAAGATGGTGATGGGATTTC-3’

NF-κβ: Nuclear factor kappa beta, TNF-α: Tumor necrosis factor-alpha, IL-6: Interlukin-6, COX: Cyclooxygenase-2, iNOS: Inducible nitric oxide synthase, GAPDH: Glyceraldehyde-3-phosphate dehydrogenase