FIG. 3.

ARE-TRP5-lacZ expression supported by diamide-hyperresistant mutants with mutations in the c-CRD region. (A) The sequence of the C-terminal 53 amino acids of Yap1p is shown in the single-letter amino acid code. The numbers refer to the position of each amino acid along the 650-residue length of Yap1p, and the locations of the three CSE repeats are indicated by underlining. The highlighted region corresponds to a putative nuclear export signal as suggested previously (29). The position and sequence of each mutant analyzed here are indicated above the amino acid sequence. (B) The ability of each mutant to regulate gene expression was assayed by introducing low-copy-number plasmids expressing the indicated forms of Yap1p into a Δyap1 strain containing the ARE-TRP5-lacZ reporter gene, as described for Fig. 1. Levels of ARE-dependent β-galactosidase activity were determined in the unstressed cells (No Stress) and in cells subjected to diamide- or H₂O₂-induced oxidative stress.