TABLE 1.

H₂O₂-mediated activation of the TRX2 gene requires normal Yap1p function

YAP1 allelea	TRX2-lacZ expression (U/A600) withb:
	No stress	Diamide	H2O2
Δyap1	3 ± 0.2	2 ± 0.3	3 ± 0.4
YAP1	6 ± 0.5	32 ± 3	31 ± 1
2μm YAP1	40 ± 5	45 ± 12	52 ± 12
CSE629AAA	13 ± 0.4	21 ± 1	13 ± 1
C629A	9 ± 0.4	9 ± 1	8 ± 1
V616D	16 ± 1	19 ± 0.4	12 ± 0.3
Δ627–3′UTR	12 ± 0.6	21 ± 1	13 ± 1
Δ220–243	6 ± 0.1	38 ± 1	40 ± 6
Δ220–335	3 ± 0.1	17 ± 0.4	8 ± 1
C303A	4 ± 0.1	15 ± 1	6 ± 0.1

^aYSC18 cells (relevant genotype: ura3-52 yap1-Δ2::hisG TRX2-lacZ) were transformed with the indicated YAP1 alleles carried on plasmids. In all cases except for the 2μm YAP1 allele, these Yap1p derivatives were expressed from low-copy-number plasmids. 

^bTransformants were grown in SC medium (20) and then left untreated or stressed by the addition of diamide or H₂O₂. TRX2-dependent β-galactosidase activity was determined as described previously (6).