TABLE 2.
Analysis of the interaction between DOK proteins and the insulin receptor in the yeast two-hybrid systema
| Plasmid | β-Galactosidase activity when cotransformed with plasmid:
|
||||
|---|---|---|---|---|---|
| IR-C | IR-CKR | pSH2-Tyro3 | pSH2-Axl | AGP4 | |
| pGD-DOKL | + | − | − | − | +++ |
| pGD-DOKLR209A | − | − | − | − | +/− |
| pGD-p62dok | + | − | − | − | +++ |
| pGD-p62dok R207A | − | − | − | − | +/− |
| pGD-Grb2 | − | − | +++ | +++ | − |
| pGD-Vav p95 | − | − | +++ | +++ | − |
Plasmids in the heading contained different DNA fragments in frame with the LexA DNA-binding domain. Plasmids in the left column contained different DNA fragments in frame with the Gal4 activation domain. Yeast strain CTY 10-5d was transformed with various pairs of plasmids, followed by an X-Gal staining assay. The strength of interaction was indicated by the timing and intensity of blue color. +++, dark blue visible in 1 h; +, blue visible in 6 h; +/−, pale blue visible overnight; −, white overnight. IR-C represents the intracellular region of the insulin receptor, and IR-CKR represents the intracellular region of the insulin receptor with the defective kinase activity.