Fig. 4.

COX inhibition during the e.c. sensitization or i.n. challenge phases contributed to the promotion of Th2/Th9/Th17 expansion in bronchial DLNs after the i.n. challenge modestly or largely, respectively. Mice were administered with indomethacin or vehicle during e.c. sensitization, i.n. challenge, or both phases. All the animal groups were e.c. sensitized to 10 mg/mL papain via intact ear skin and subsequently i.n. challenged (2.5 μg papain). a Time line. b Bronchial DLN cell cytokine responses restimulated in vitro. Data are indicated as the mean ± SD of 4 wells of pooled DLN cells from 5 or 4 mice per group and are representative of 3 independent experiments with similar results. Detection limits are indicated by dotted lines. *p < 0.05 by ANOVA among the 4 animal groups (vs. no indomethacin). ^#p < 0.05 by ANOVA among the 4 animal groups (vs. medium). COX, cyclooxygenase; DLN, draining lymph node; e.c., epicutaneous.