FIG. 6.

P-selectin secretion is impaired in LAT-deficient mouse platelets in response to CRP. Washed platelets were stimulated by incubating with CRP for 2 min and then treated with anti-P-selectin and FITC-conjugated secondary antibody for 10 min. Ten thousand events were acquired for each samples. Responsive cells are counted as positive events by delimiting a threshold between basal and stimulated platelets; the threshold was set at a position corresponding to the 98th percentile of the population (dotted line). (a) Histograms of fluorescence corresponding to basal and CRP- and thrombin (Thr)-stimulated platelets for control (+/+) and LAT-deficient (−/−) mouse platelets. The results are from one experiment representative of three. (b) Concentration-response curve of P-selectin secretion in response to CRP. The results are from one experiment representative of three.