FIG. 7.

Fibrinogen binding is decreased in LAT-deficient mouse platelets in response to CRP. Platelets were stimulated with CRP for 2 min in the presence of their own diluted platelet-poor plasma (to replace fibrinogen). Aliquots (50 μl) were incubated with FITC-conjugated anti-fibrinogen antibody for 30 min. Samples were fixed with 1% formaldehyde and analyzed by using a one-fluorescence-channel setting. (a) Histograms of fluorescence for control (+/+) and Lat-deficient (−/−) platelets in the basal state (thin line) or stimulated with 3 μg of CRP per ml (thick line). (b) Concentration-response curve of fibrinogen binding in response to CRP. The y axis denotes the geometric mean of the fluorescence. Results are from one experiment representative of three.