TABLE 3.

Sequences of serovar-specific primer pairs for real-time PCR.

Serovar	Name	Primer sequences (5′→3′)	Size (bp)
Aberdeen	Aberdeen-F	AAC AAC GGG TAC AGG GAT TA	131
	Aberdeen-R	AAT CCT TAT TAT CGT CCC CA
Agona	Agona-F	GCA TCT GGC GGT AAG TCA TA	190
	Agona-R	GTG AGC GTA ATG GGG ATG TA
Albany	Albany-F	TAG TCA GGT AGC ACC GAG TT	163
	Albany-R	ACG CCA TGT AGA TTC GTT AT
Anatum	Anatum-F	AAA GCA CCC TGA GTC AGA TG	143
	Anatum-R	CAA GTC CAC CGA CTG CTC TA
Bareilly	Bareilly-F	GGT GGT AGT ACC AAG GAT GT	114
	Bareilly-R	ACT GCT AGT TCC TCC GTA AG
Berta	Berta-F	TAA CCG AGG AGC CAA CAG TG	145
	Berta-R	CGG AGA GGG TCC AGT TGT TT
Blockley	Blockley-F	TCC GTG GTT CAT GAG CAG TT	124
	Blockley-R	GAA GGT CAT CAC GCC TAG GT
Braenderup	Braenderup-F	GGA GAA TGC TTG CAG GAA GA	156
	Braenderup-R	GCT GGT TCA AAG TAA TGC GG
Brandenburg	Brandenburg-F	TGG TTC TAC ACC TAA AGG TGG	108
	Brandenburg-R	TAC GCG ACA TCA TCT AGC CT
Cerro	Cerro-F	CGT TTC TCC GTT TAT GTG GA	157
	Cerro-R	GGC ATT GTT ACA GAC AAA GC
Choleraesuis	Choleraesuis-F	GCT CCA TCT TCG CCA ATT GA	125
	Choleraesuis-R	TCC AGT AAC GCT GTA GGC TCT
Corvallis	Corvallis-F	AAG CGT TTA TTG GAG GCT GA	139
	Corvallis-R	CGC TGT TCG ATG CTT CAA GT
Derby	Derby-F	TGC GTC CGT TGT TCA ATG TG	106
	Derby-R	CGT TGT ACG CCA TTC AGC TT
Dublin	Dublin-F	GCG TCA AGG TTT ATT GAA TCG	114
	Dublin-R	GGA TGT CAA TCG CTG TTG TC
Elisabethville	Elisabethville-F	GAC CAC GAC CGG TAC AGC AA	178
	Elisabethville-R	CGG CGG ATA CTG CAC ACG AA
Enteritidis	Enteritidis-F	TTG GTA AAT CCG TCG GAC AA	105
	Enteritidis-R	AAT CGC TAC GCG CCT CAA TA
Gallinarum	Gallinarum-F	CGA CGG TCG TCA ATC CTA CT	113
	Gallinarum-R	ATC AAC CAC AGC CGT AGC AG
Give	Give-F	TCA TTG GCA CTG GTG AGT CG	103
	Give-R	CCT TCA ATG CCT GGC ACA TC
Hadar	Hadar-F	GTG AGT CTT TTT CGG TGA TA	162
	Hadar-R	ATC TCA CCC ATT CAC AGA TA
Heidelberg	Heidelberg-F	CGG CGA ATT AAT CAT AAG CG	105
	Heidelberg-R	CTC TCA CCT GAT TTT GCC TGT
I 4,[5],12:i:-	I 4,[5],12:i:-F	AAG TGC GCC AGT TAG CTT CT	113
	I 4,[5],12:i:-R	GGT ATC GCC GTC AAT ACA CA
Infantis	Infantis-F	GGT CGA GAT GGG TAT GTA GC	109
	Infantis-R	CAG GAG TTC CTG CGC AAC CA
Javiana	Javiana-F	TGG CTA CTC AGG CAG TAC TA	112
	Javiana-R	AGC ATA ACT CCG TGA GTT TT
Kedougou	Kedougou-F	GTC AGC CTT CCT GAA GTC AT	102
	Kedougou-R	CTC GCG TTC ATA CAA TCC TG
Kentucky	Kentucky-F	CGA TAA CTT GCG GAG TGA CA	141
	Kentucky-R	TTC CAC CGT TGG CCT CAT AA
Kottbus	Kottbus-F	GCG TCT GAC TGG AGC AGA TT	108
	Kottbus-R	ACC ACA GTC AAC GCC TAG GT
Litchfield	Litchfield-F	CAG ACT TAA TAG AGG ACC CA	143
	Litchfield-R	CTC CGT TTC ATT CCA TCC AC
Livingstone	Livingstone-F	TCT GCG CAC AGG CGA ATT CT	103
	Livingstone-R	CAG ACG CTT AGA GAC GGT GTG A
London	London-F	GGC TCA TCC GGA ACG AAC AA	141
	London-R	CAA GCG AGC TTA TAG GCG TAG
Manhattan	Manhattan-F	GCT GAT GCA GCG TAG CAA TA	128
	Manhattan-R	GCT CAC TAA GAA GGC ATG ACT C
Mbandaka	Mbandaka-F	ATC GAG GAT CCA AGC ATC AG	130
	Mbandaka-R	GGA AAA CAC CAA GGA CTT CG
Meleagridis	Meleagridis-F	TGG CGA TAT ACC GGT TAC CT	118
	Meleagridis-R	TCC GCG TAA CTG ATC ACT TC
Menston	Menston-F	TAG TGT TGC GAC GGA GCT AA	101
	Menston-R	TTC GAA CAG CCA GCA GTG AA
Minnesota	Minnesota-F	GCG GCT ACA AGC ATC ATC AT	117
	Minnesota-R	CCT TCC CAA CTC GAA CTT TAA C
Mississippi	Mississippi-F	CCA CGA CAC CAT CAA TCA TC	115
	Mississippi-R	GCA ATA GGC GGT ACT AAG GA
Montevideo	Montevideo-F	CCA ACC TGG CCA ACA AGA TT	120
	Montevideo-R	GAA CTG TCG CAC ACC GAT TC
Muenchen	Muenchen-F	GCA CGT ATG CAG ATC GAA GA	134
	Muenchen-R	GTT AGC CGT TCC ACT GAC AA
Muenster	Muenster–F	CAC CTC CTG AGA CTG AAG AA	175
	Muenster–R	CCG TCA TTT AGA TAA GGA AG
Newington	Newington-F	CGT AGT CGT GGT TGC TGG TA	121
	Newington-R	TGC AGC AAG TAT GAC GAA TG
Newport	Newport-F	GTT GCC AAA AAG CAC AAT GA	117
	Newport-R	AGC TCG AGT AAT CCG CAT GA
Ohio	Ohio-F	CGA TAA TTG CCG CCT TCT GA	119
	Ohio-R	TCA GCA GGA GCG TGA CAG TT
Oranienburg	Oranienburg-F	GCT GAG ATT GTG ATT CCA CC	101
	Oranienburg-R	CGC TGT TCT AACCTT GAG GA
Panama	Panama-F	GCT CAA TTA GAT CCA ACA GC	104
	Panama-R	GAC TGG AGT GCA AGG TAG TT
Paratyphi B	Paratyphi B-F	CGA TGG CTC GAT CCT GTT CAA G	120
	Paratyphi B-R	GTC CGG CGG ACA ACT ATC AAC C
Poona	Poona-F	TGT TGG AGG ATG CCA TGA GT	127
	Poona-R	AAG GAC AGC TTG CGT ATG GA
Reading	Reading-F	GCG AAT GGC GAT AAG GTT GA	128
	Reading-R	GCT CCG ATC AAA ACA TGA GTC
Rissen	Rissen-F	GAG CTA GTT GCC GAA TCG AA	117
	Rissen-R	CCG AAT GAA TGC TGG CAA GT
Saintpaul	Saintpaul-F	TGA TGG GAT ATC TCG CAA CA	154
	Saintpaul-R	GCC GCT ATG GAA CTT ATT CG
Schwarzen- grund	Schwarzen- grund-F	TGG CGC TAT TAC CAC TGA TG	131
	Schwarzen- grund-R	CAA TTG CTG CGG ACC AAC TA
Senftenberg	Senftenberg-F	GAG CAG GAT ATG CGC GAC TA	131
	Senftenberg-R	GTT GCT GCT CCA TGG TGT TG
Singapore	Singapore-F	CCA GCA GAT AGG AAC ATA GG	109
	Singapore-R	GGA ACG ATA AGG CAT CAT CA
Stanley	Stanley-F	TAC TGG CCT GGT GTC TTG TT	120
	Stanley-R	GTA TCC ATT GCC AGC GAG TA
Tennessee	Tennessee-F	ACA AAC AAG CCT TCA GGT GG	102
	Tennessee-R	CAG CTC CTT CTG TTG CTC AT
Thompson	Thompson-F	TAT TGC AAC AAT GAG GCC CTC T	126
	Thompson-R	GTC GCG ATT CTG AAC CGT GTC
Typhi	Typhi-F	TGA GGC ACA CCG TGA TGA ACT G	118
	Typhi-R	ATT ATC CGC TCC GCG AAT GCT
Typhimurium	Typhimurium-F	CGG CGG TAA TAC GAT GAA CT	126
	Typhimurium-R	CTT GCT GTC AGT GCT GTC TT
Uganda	Uganda-F	AAG ATG TCT GGC ATG GGC AA	106
	Uganda-R	CGG GCT CCA CCA ACA AAA TG
Vinohrady	Vinohrady-F	GAA GGA TTG CGA TTC GCT TT	117
	Vinohrady-R	GGA TGA CAT CAG CGA GTT CT
Virchow	Virchow-F	GCC ACT GAT GAG ATG GAG TA	172
	Virchow-R	ACT CGC CAT CAG CAA TAC AC
Weltevreden	Weltevreden-F	AAC CGG ATC CTG AGC CAT AC	111
	Weltevreden-R	CCG CTG CAA TAG CTG ATC TT
Internal standard1	U bacteria-F	ATG TTG GGT TAA GTC CCG	265–267
	U bacteria-R	CTA GCG ATT CCR RCT TCA

¹Internal standard primer pair was developed by Aboutalebian et al. (2021).