Table 8.
Precision and accuracy of 8 compounds in rat plasma (n = 6).
| Compounds | Spiked concentration (ng/mL) |
Intra-day |
Inter-day |
||||
|---|---|---|---|---|---|---|---|
| Measured (ng/mL) |
RE (%) |
RSD (%) |
Measured (ng/mL) |
RE (%) |
RSD (%) |
||
| Oleuropein | 0.5 | 0.4 ± 0.1 | –13.3 | 11.9 | 0.5 ± 0.1 | –4.4 | 13.5 |
| 5 | 4.9 ± 0.3 | –3.0 | 5.2 | 5.1 ± 0.4 | 2.6 | 7.9 | |
| 100 | 100.8 ± 7.0 | 0.8 | 6.9 | 108.9 ± 10.1 | 8.9 | 9.3 | |
| Chlorogenic acid | 1 | 1.1 ± 0.1 | 13.3 | 4.6 | 1.1 ± 0.1 | 12.8 | 5.9 |
| 10 | 9.0 ± 0.5 | –10.0 | 5.3 | 9.4 ± 0.6 | –6.1 | 6.0 | |
| 200 | 193.2 ± 6.5 | –3.4 | 3.4 | 209.6 ± 15.7 | 4.8 | 7.5 | |
| Formononetin | 1 | 1.1 ± 0.1 | 11.7 | 8.8 | 1.1 ± 0.1 | 9.4 | 8.6 |
| 10 | 10.3 ± 0.3 | 3.2 | 2.8 | 10.3 ± 0.4 | 3.2 | 4.1 | |
| 200 | 224.4 ± 6.5 | 12.2 | 2.9 | 221.8 ± 11.2 | 10.9 | 5.0 | |
| Verbascoside | 2 | 2.0 ± 0.1 | 1.7 | 6.0 | 2.0 ± 0.2 | 2.2 | 7.4 |
| 20 | 21.2 ± 1.3 | 5.8 | 6.2 | 21.6 ± 1.4 | 8.1 | 6.6 | |
| 400 | 424.0 ± 22.5 | 6.0 | 5.3 | 419.5 ± 16.4 | 4.9 | 3.9 | |
| Linarin | 1 | 1.0 ± 0.1 | 3.3 | 10.0 | 1.1 ± 0.1 | 6.1 | 10.3 |
| 10 | 10.2 ± 0.4 | 1.7 | 3.8 | 10.4 ± 0.3 | 3.8 | 3.2 | |
| 200 | 213.1 ± 4.1 | 6.5 | 1.9 | 217.3 ± 12.1 | 8.7 | 5.5 | |
| Luteolin | 1 | 1.1 ± 0.1 | 11.7 | 8.8 | 1.1 ± 0.1 | 9.4 | 8.6 |
| 10 | 10.0 ± 0.3 | –0.5 | 3.0 | 10.3 ± 0.5 | 3.0 | 4.8 | |
| 200 | 221.7 ± 7.1 | 10.8 | 3.2 | 221.0 ± 8.9 | 10.5 | 4.0 | |
| Jaspolyside | 1 | 1.1 ± 0.1 | 5.0 | 11.7 | 1.0 ± 0.1 | 2.8 | 11.0 |
| 10 | 9.7 ± 1.0 | –2.8 | 10.6 | 9.6 ± 0.8 | –4.2 | 8.6 | |
| 200 | 200.3 ± 17.6 | 0.1 | 8.8 | 209.5 ± 16.5 | 4.8 | 7.9 | |
| Specnuezhenide | 1 | 1.1 ± 0.1 | 10.0 | 10.0 | 1.1 ± 0.1 | 6.7 | 10.2 |
| 10 | 10.3 ± 0.5 | 3.0 | 4.8 | 10.3 ± 0.5 | 3.1 | 5.2 | |
| 200 | 213.7 ± 7.6 | 6.9 | 3.6 | 215.0 ± 9.2 | 7.5 | 4.3 | |