FIG. 3.

Deletion or disruption of the first N-terminal coiled-coil domain (CC1) enhances Myr-Fes transforming activity in Rat-2 cells. Wild-type Fes, Fes with an N-terminal myristylation signal (Myr-Fes), and Myr-Fes with insertion (Myr-βCC1) or deletion (Myr-ΔCC1) mutations in the CC1 domain were introduced into Rat-2 fibroblasts by using recombinant retroviruses. Infected cells were selected with G418 for 2 weeks and observed for the appearance of transformed foci. (A) After Wright-Giemsa staining, foci from three independent experiments were counted daily from day 10 to 14, and the average of the results is shown (± the standard deviation [SD]). (B) The average focus size was also determined for each culture under low-power microscopy. Results shown are the average values for three independent experiments ± the SD. Symbols: ○, c-Fes; □, Myr-Fes; ●, Myr-βCC1; ■, Myr-ΔCC1.