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[Preprint]. 2021 Sep 29:rs.3.rs-926789. [Version 1] doi: 10.21203/rs.3.rs-926789/v1

Figure 4.

Figure 4

MBaMV infects human monocyte-derived macrophages (MDM) in a CD150-independent manner. a-b, MDMs were infected with EGFP-reporter MeV or MBaMV (1×105 IU/sample) and were either (a) fixed by 2% PFA at 24 hpi, DAPi-stained and imaged (scale bar is 200 μm), or (b) quantified by flow cytometry. The percent of CD68+GFP+ MDMs from 6 donors are shown. Open and crossed symbols indicate experiments using lot 1 and lot 2 viruses, respectively. Adjusted p values are from one way ANOVA with Dunnett’s multiple comparisons test. c, Soluble human CD150 (sCD150) or a dimeric Fc fusion construct (sCD150-Fc) inhibited MeV but not MBaMV infection of macrophages. GFP+ events in untreated controls were set to 100%, and entry under sCD150/sCD150-Fc were normalized to untreated controls. Adjusted p values are from two-way ANOVA with Šidàk’s multiple comparisons test. In (b) and (c), data shown are mean +/− S.D. from multiple experiments (N=5–7) with individual values also shown. (d) Exemplar FACS plots from the summary data shown in (b). R2 (GFP-dim) and R3 (GFP-bright/CD150-low) gates are indicated as described in the text. e, ConA/IL-2 stimulated PBMCs were infected with MeV or MBaMV (MOI of 0.1) and analyzed for GFP expression by flow cytometry at 24 hpi.