Fig 2. Cytosolic access is necessary for L. monocytogenes-stimulated PGE₂ production in vivo.

C57BL/6 mice were infected with 10⁵ wild-type or 10⁷ Δhly L. monocytogenes. 12hpi spleens were harvested for eicosanoid extraction and mass spectrometry (A) and livers were harvested for bacterial burdens (B). C57BL/6 mice were infected with 10⁵ wild-type, 10⁷ Δhly L. monocytogenes, or a combination of both strains. 12hpi spleens were harvested for eicosanoid extraction and mass spectrometry (C). Data are representative of two independent experiments. Mass spectrometry data was normalized to d-PGE₂ levels and fold change is compared to PBS controls. Significance was determined by a one-way ANOVA with Bonferroni’s correction. **p < 0.01, ****p < 0.0001.