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. 2021 Sep 10;10:e71335. doi: 10.7554/eLife.71335

Figure 4. Tissue-specific effects after DAF-2 depletion on lifespan extension and reactive oxygen species resistance.

Figure 4.

(A) Auxin treatment extended lifespan in aged DAF-2::degron animals. Animals were shifted from DMSO plates to plates containing 1 mM auxin on the indicated day. (B) Auxin-mediated depletion of DAF-2 enhanced oxidative stress resistance to a similar extent as daf-2(e1370) animals. Animals were shifted as L4 for 24 hr on plates containing DMSO or 1 mM auxin at 20°C. (C) Intestinal knockdown of DAF-2 enhanced oxidative stress resistance. Animals were shifted to DMSO or 1 mM auxin plates at the L4 stage and kept until day 5 of adulthood at 20°C. (D) Quantification of (C) from three independently performed experiments. Error bar represents s.d. *: p < 0.05, ***: p < 0.001. (E) skn-1 was necessary for full lifespan extension in DAF-2 depleted animals at 15°C. Animals were kept on 1 mM auxin. p < 0.0001. (F) Knockdown of skn-1 extended the mean lifespan of DAF-2 depleted animals at 25°C. Animals were raised at 15°C and shifted to 1 mM auxin at 25°C. p < 0.0001. For (A–F), see Source data 1 and Source data 3 for raw data and Supplementary file 1 for statistics and additional trials.