Key resources table.

Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
Strain, strain background (Mus musculus, male and female)	CD-1	Charles River	Strain code 022
Recombinant DNA reagent	Library1	This paper		Listed in Supplementary file 1
Recombinant DNA reagent	Library2	This paper		Listed in Supplementary file 2
Recombinant DNA reagent	pJK01_Rhominprox-DsRed	Kwasnieski et al., 2012	AddGene plasmid # 173,489
Recombinant DNA reagent	pJK03_Rho_basal_DsRed	Kwasnieski et al., 2012	AddGene plasmid # 173,490
Sequence-based reagent	Primers	IDT		Listed in Supplementary file 6
Commercial assay or kit	Monarch PCR Cleanup Kit	New England Biolabs	T1030S
Commercial assay or kit	Monarch DNA Gel Extraction Kit	New England Biolabs	T1020L
Commercial assay or kit	TURBO DNA-free	Invitrogen	AM1907
Commercial assay or kit	SuperScript III Reverse Transcriptase	Invitrogen	18080044
Software, algorithm	Bedtools	https://bedtools.readthedocs.io/en/latest/	RRID:SCR_006646
Software, algorithm	MEME Suite	https://meme-suite.org/	RRID:SCR_001783
Software, algorithm	ShapeMF	https://github.com/h-samee/shape-motif, Samee, 2021	DOI:10.1016/j.cels.2018.12.001
Software, algorithm	Numpy	https://numpy.org/	DOI:10.1038/s41586-020-2649-2
Software, algorithm	Scipy	https://www.scipy.org/	DOI:10.1038/s41592-019-0686-2
Software, algorithm	Pandas	https://pandas.pydata.org/	DOI:10.5281/zenodo.3509134
Software, algorithm	Matplotlib	https://matplotlib.org/	DOI:10.5281/zenodo.1482099
Software, algorithm	Logomaker	https://github.com/jbkinney/logomaker, Justin, 2021	DOI:10.1093/bioinformatics/btz921