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. 2021 Oct 5;10:e69207. doi: 10.7554/eLife.69207

Figure 4. Time course of mitochondria dynamics and actin accumulation at the mitochondrial fission site under siDRP1.

(A) Representative live cell images of mitochondria with GJA1-20k under siDRP1. (B) Representative mitochondrial dynamics in GJA1-20k-transfected cells by time-lapse live cell imaging under siDRP1 treatment. The bottom images (with fire look-up table) indicate the product of GJA1-20k and actin signals. The white lines indicate mitochondrial outlines. Scale bars, 5 μm (A) and 2 μm (B). (C) The intensity of mitochondria and the product of GJA1-20k and actin from (B). The colored areas indicate fission sites along the lines shown in each respective insert. (D–F) The time course of the product of GJA1-20k and actin intensity (D), mitochondrial intensity (E), or combined relative intensity (F) at the fission site from the mitochondrion shown in (B). Curves are four parameters logistic (4PL) fits to the data. Time 0 corresponds to the peak product of actin and GJA1-20k intensity. The arrows indicate the time point at which fission is complete. (G) Measured time from peak product of actin and GJA1-20k intensity to fission in seconds (bars indicate median and 95 % confidence interval). n = 30 events. All data points are provided in the source data.

Figure 4—source data 1. All data points of the intensity and time-to-fission for Figure 4.

Figure 4.

Figure 4—figure supplement 1. Time course of mitochondria dynamics and actin acumulation at the mitochondrial fission site under Mdivi-1 treatment.

Figure 4—figure supplement 1.

(A) and (C) Representative mitochondrial dynamics in GJA1-20k-transfected cells by time-lapse live cell imaging under Mdivi-1 treatment. The bottom images (with fire look-up table) indicate the product of GJA1-20k and actin signals. The white lines indicate mitochondrial outlines. Scale bars, 2 μm. (B) and (D) The intensity of mitochondria and the product of GJA1-20k and actin from (A) and (C), respectively. The colored areas indicate fission sites along the lines shown in each respective insert. (E–G) The time course of the product of GJA1-20k and actin intensity (E), mitochondrial intensity (F), or combined relative intensity (G) at the fission site from the mitochondrion shown in (A). Curves are four parameters logistic (4PL) fits to the data. Time 0 corresponds to the peak product of actin and GJA1-20k intensity. The arrows indicate the time point at which fission is complete. (H) Measured time from peak product of actin and GJA1-20k intensity to fission in seconds (bars indicate median and 95% confidence interval). n = 29 events. All data points are provided in the source data.
Figure 4—figure supplement 1—source data 1. All data points of All data points of the intensity and time-to-fission for Figure 4—figure supplement 1.