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. 2021 Sep 17;10:e67670. doi: 10.7554/eLife.67670

Figure 3. Endogenous ciliary membrane proteins are sorted to ectosomes and exported to AMsh.

Figure 3.

(A) GCY-8-wrmScarlet was cell-specifically expressed in AFD (driven by gcy-8 promoter). GCY-8-wrmScarlet is enriched in AFD microvilli and AFD periciliary membrane compartment (PCMC). GCY-8-wrmScarlet-carrying extracellular vesicles (EVs) were also observed within AMsh cytoplasm, in the vicinity of the AFD nerve receptive endings and in AMsh cell body (magenta arrowheads). (B) SRTX-1-wrmScarlet was cell-specifically expressed in AFD (driven by gcy-8 promoter). SRTX-1-wrmScarlet is enriched in AFD microvilli and PCMC, similarly to GCY-8. Within AMsh cytoplasm, SRTX-1-wrmScarlet-carrying EVs are observed in the vicinity of the AFD neuron receptive endings and in AMsh cell body (magenta arrowheads). (C) SRBC-64-wrmScarlet was cell-specifically expressed in ASK neurons (driven by srbc-64 promoter). SRBC-64-wrmScarlet is observed in the ASK cilia proper (C) and PCMC but not in the cytoplasm of AMsh. (D) GCY-22-wrmScarlet was cell-specifically expressed in ASER (driven by gcy-5 promoter). GCY-22-wrmScarlet is observed in ASER cilium tip and in ASER PCMC. ASER PCMC shows a rounded protrusion, which we consider as a recently excised EV (orange arrowhead). Within AMsh cytoplasm, a GCY-22-wrmScarlet-containing ectosome is located in the vicinity of the ASER PCMC. Few but large GCY-22-wrmScarlet-carying EVs are observed in AMsh cell body. Scale bar: 20 μm in head images, 5 μm in insets.