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. 2021 Sep 17;10:e67670. doi: 10.7554/eLife.67670

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© 2021, Razzauti and Laurent

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Author response image 1. — ced-10(n3246) did not affect neuronal uptake of DiI nor the DiI export from ciliated neurons to AMsh glia. AMsh cell body position (dashed line is aligned to the position of the head ganglia) and projections (green arrows) are modified in ced-10(n3246) mutants. In ced-10(n3246), neurons identified as ASI and ASJ are displaced posteriorly and/or show abnormal neurite extensions (B) The AMsh cell body position relative to the nose tip of the animals was measured for AMsh::DYN-1(K46A) and for ced-10(n3246). (C) We quantified the number of GCY-22-wrmScarlet carrying vesicles within AMsh in animals overexpressing GCY-22-wrmScarlet in ASER. Their number was increased in animals expressing AMsh::DYN-1(K46A) compared to controls. ced-10(n3246) and alx-1(gk338) did not modify the number of GCY-22-wrmScarlet carrying vesicles in AMsh.