Video 1. Extracellular vesicles (EVs) are released from the DiI-uptaking amphid neurons and are captured by the surrounding glia.

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An animal expressing AMsh cytoplasmic CFP and stained with DiI was immobilized with 10 mM tetramisole and recorded during approximately 8 min. Membrane fragments become detached from ciliary regions; these DiI-carrying vesicles are sequentially trapped by glial cells (orange arrows). Dashed arrow indicates directional flow of vesicles towards AMsh cell body. AMsh ‘pocket’ is indicated and contains multiple already-exported vesicles. Scale bar: 5 μm.