Video 6. Budding of basal ectosomes containing GCY-22-wrmScarlet originating from ASER cilium.

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An animal expressing GCY-22-wrmScarlet in ASER was immobilized with 10 mM tetramisole and recorded during 12 min. The acquisition plane corresponds to ASER periciliary membrane compartment (PCMC), for informative purposes ASER cilium location is indicated. Large basal ectosomes (∼1 μm diameter) carrying GCY-22-wrmScarlet (magenta) are observed being released from the PCMC of ASER, released material ends up in AMsh cell (blue, AMsh::CFP cytoplasmic expression). GCY-22-wrmScarlet channel is shown in inverted LUT on the left. Scission events occur at t = 4 s and t = 348 s (orange arrow indicates the start of each scission events). Scale bar: 5 μm.