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. 2021 Sep 17;10:e67670. doi: 10.7554/eLife.67670

Video 7. Budding of apical ectosomes containing GCY-22-GFP originating from ASER cilia tip.

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Four examples of bbs-8(nx77) mutants expressing endogenously GCY-22-GFP. Each animal was immobilized with 10 mM tetramisole and recorded between 30 s and 4.5 min. Ectosomes shed continuously from ASER cilia tip (orange arrowheads) are sequentially released to the environment. Scale bar: 5 μm.