Video 8. Budding of apical ectosomes containing TSP-6-wrmScarlet originating from one or several cilia of ASK, ADF, ASJ, ASI, and ASH.

Download video file ^{(1,003.9KB, mp4)}

An animal expressing endogenous TSP-6 levels (tsp-6:wrmScarlet knock-in strain) was immobilized with 10 mM tetramisole and recorded during approximately 2,5 min. Ectosomes shed continuously from the cilia tip (white arrowheads) and are trapped within the pore channel occasionally being released to the environment. Scale bar: 5 μm.