Table 1. Data collection, processing, refinement, and model statistics.
| Data collection | |
|---|---|
| Voltage (kV) | 300 |
| Pixel size (Å) | 1.04 |
| Detector | Falcon III |
| Defocus range (μm) | −1.1 to −3.2 |
| Defocus mean (μm) | −2.1 |
| Electron dose (e− frame−1 Å−2) | 1.5 |
| Data processing | |
| Independent data collections | 7 |
| Useable micrographs | 43,950 |
| Particles picked | 3,374,367 |
| Final particles | 66,340 |
| Map sharpening B-factor (Å2) | −10 |
| Resolution (Å) | 3.4 |
| EMPIAR accession code | EMPIAR-10809 |
| EMDB accession code | EMD-13329 |
| PDB accession code | 7PD3 |
| Model composition | |
| Chains | 70 |
| Non-hydrogen atoms | 108,053 |
| Protein residues | 9276 |
| RNA bases | 1522 |
| Metals (Mg2+/Zn2+) | 91/2 |
| Ligands | GCP (1), SAM (1) |
| Refinement | |
| Resolution (Å) | 3.4 |
| CC (mask) | 0.70 |
| R.M.S deviations | |
| Bond lengths (Å) | 0.009 |
| Bond angles (°) | 1.068 |
| Validation | |
| Molprobity score | 1.66 |
| Clashscore, all atoms | 4.97 |
| Rotamers outliers (%) | 0.01 |
| Cβ outliers (%) | 0.01 |
| Ramachandran plot | |
| Favored (%) | 94.17 |
| Allowed (%) | 5.69 |
| Outliers (%) | 0.14 |