Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Pseudomonas aeruginosa) | PA14 | Rahme et al., 1995; PMID:7604262 | ||
| Strain, strain background (P. aeruginosa) | PA14 ∆orn | This study | ||
| Strain, strain background (Escherichia coli) | Stellar cells | Takara/Clontech | ||
| Strain, strain background (E. coli) | BL21(DE3) | New England Biolabs | ||
| Recombinant DNA reagent | pEX-Gn-∆orn (plasmid) | This study | ||
| Recombinant DNA reagent | pJN105 (plasmid) |
Newman and Fuqua, 1999; PMID:10023058 |
||
| Recombinant DNA reagent | pJHA (plasmid) | Kim et al., 2019; PMID:31225796 | ||
| Sequence-based reagent | ACAGATTGGTGGATC CATGACCGAAATTCGCG TGCATCAGGGCGATCTGC CGAACCTGGATAACTAT CGCATTGATG CGGTGGCG GTGGATACCG AAACCCTGG GCCTGCAGCC GCATCGCGAT CGCCTGTGCG TGGTGCAGCTG AGCAGCGGCGA TGGCACCGC GGATGTGATTCA GATTGCGAA AGGCCAGAAAA GCGCGCCGAA CCTGGTGCGCC TGCTGAGCG ATCGCGATATT ACCAAAATTTTT CATTTTGGCCGC TTTGATCTG GCGATTCTGGCG CATACCTTTG GCGTGATGCCG GATGTGGTGT TTTGCACCAAAAT TGCGAGCAA ACTGACCCGCAC CTATACCGATCGC CATGGCCTGAAAG AAATTTGCGG CGAACTGCTGAAC GTGAACATTAG CAAACAGCAGCAG AGCAGCGATTG GGCGGCGGAAAC CCTGAGCCGCG CGCAGATTGAATAT GCGGCGAGCG ATGTGCTGTATCTG CATCGCCTGAA AGATATTTTTGAAG AACGCCTGAAA CGCGAAGAACGCG AAAGCGTGGCG AAAGCGTGCTTTC AGTTTCTGCCGA TGCGCGCGAACC TGGATCTGCTGG GCTGGAGCGAAATTG ATATTTTTGCG CATAGCTAAGCGGC CGCACTCGAGCA (DNA fragment) |
Geneart | BH02530 | Custom DNA fragment for Bartonella henselae NrnC, cloned in to His6-SUMO-pET28 |
| Sequence-based reagent | ACAGATTGGTG GATCCATGACCA TTCGCTTTCATC GCAACGATCT GCCGAACCTGGA TAACTATCAGG TGGATGCGGTG GCGATTGATAC CGAAACCCTGG GCCTGAACCCGC ATCGCGATCGCC TGTGCGTGGTG CAGATTAGCCCG GGCGATGGCAC CGCGGATGTGA TTCAGATTGAAGC GGGCCAGAAAAAA GCGCCGAACC TGGTGAAACTGC TGAAAGATCGC AGCATTACCAAAA TTTTTCATTTTG GCCGCTTTGATC TGGCGGTGCTG GCGCATGCGTTT GGCACCATGCC GCAGCCGGTGTT TTGCACCAAAAT TGCGAGCAAACTG ACCCGCACCT ATACCGATCGCCAT GGCCTGAAAG AAATTTGCAGCGA ACTGCTGGATG TGAGCATTAGCAA ACAGCAGCAG AGCAGCGATTGGG CGGCGGAAG TGCTGAGCCAGG CGCAGCTGGAA TATGCGGCGAG CGATGTGCTGTAT CTGCATCGCCTG AAAGCGGTGCT GGAACAGCGCCT GGAACGCGAT GGCCGCACCAAA CAGGCGGAAGC GTGCTTTAAATTT CTGCCGACCCG CAGCGAACTGGA TCTGATGGGCTG GGCGGAAAGCGA TATTTTTGCGCAT AGCTAAGCGGCCGCACTC GAGCA (DNA fragment) |
Geneart | BMEI1828 | Custom DNA fragment for Brucella melitensis NrnC, cloned in to His6-SUMO-pET28 |
| Recombinant DNA reagent | His6-SUMO-pET28- NrnCBh (plasmid) |
This study | Cloned from custom DNA fragment | |
| Recombinant DNA reagent | His6-SUMO-pET28- NrnCBm (plasmid) |
This study | Cloned from custom DNA fragment | |
| Recombinant DNA reagent | pJHA-NrnCBh (plasmid) | This study | NrnCBh cloned into pJHA for expression in P. aeruginosa | |
| Recombinant DNA reagent | pJHA-NrnCBm (plasmid) | This study | NrnCBm cloned into pJHA for expression in P. aeruginosa | |
| Recombinant DNA reagent | pJHA-NrnCBh
D25A (plasmid) |
This study | Product of site-directed mutagenesis of pJHA-NrnCBh | |
| Recombinant DNA reagent | pJHA-NrnCBh
E27A (plasmid) |
This study | Product of site-directed mutagenesis of pJHA-NrnCBh | |
| Recombinant DNA reagent | pJHA-NrnCBh
D84A (plasmid) |
This study | Product of site-directed mutagenesis of pJHA-NrnCBh | |
| Recombinant DNA reagent | pJHA-NrnCBh
D155A (plasmid) |
This study | Product of site-directed mutagenesis of pJHA-NrnCBh | |
| Recombinant DNA reagent | pJHA-NrnCBh
Y151A (plasmid) |
This study | Product of site-directed mutagenesis of pJHA-NrnCBh | |
| Recombinant DNA reagent | pJHA-NrnCBh
L31A (plasmid) |
This study | Product of site-directed mutagenesis of pJHA-NrnCBh | |
| Recombinant DNA reagent | pJHA-NrnCBh
H79A (plasmid) |
This study | Product of site-directed mutagenesis of pJHA-NrnCBh | |
| Recombinant DNA reagent | pJHA-NrnCBh
K103A (plasmid) |
This study | Product of site-directed mutagenesis of pJHA-NrnCBh | |
| Recombinant DNA reagent | pJHA-NrnCBh
H205A (plasmid) |
This study | Product of site-directed mutagenesis of pJHA-NrnCBh | |
| Recombinant DNA reagent | pJHA-NrnCBh
K132A (plasmid) |
This study | Product of site-directed mutagenesis of pJHA-NrnCBh | |
| Sequence-based reagent | TTTGGGCTAGCCA TATGACCGAAATT CGTGTTCATCAGGG |
Life Technologies | NrnCBh_ infusionprimer_F |
Primer for cloning NrnCBh into pJHA |
| Sequence-based reagent | GCTCAAGCTTGAAT TCGCTGTGTGCAA AGATATCAATTTCG |
Life Technologies | NrnCBh_ infusionprimer_R |
Primer for cloning NrnCBh into pJHA |
| Sequence-based reagent | TTTGGGCTAGCCATATGACCATTCGTTTTCATCGTAATGATC | Life Technologies | NrnCBm_ infusionprimer_F |
Primer for cloning NrnCBm into pJHA |
| Sequence-based reagent | GCTCAAGCTTGAATTCGCTATGTGCAAAAATATCGCTTTC | Life Technologies | NrnCBm_ infusionprimer_R |
Primer for cloning NrnCBm into pJHA |
| Sequence-based reagent | Acccagtgtttcggtagc aacggcaactgcatc |
Life Technologies | NrnCBh D25A_a | Primer for site directed mutagenesis |
| Sequence-based reagent | Gatgcagttgccgttgct accgaaacactgggt |
Life Technologies | NrnCBh D25A_b | Primer for site directed mutagenesis |
| Sequence-based reagent | Gttgccgttgataccgca acactgggtctgcag |
Life Technologies | NrnCBh E27A_a | Primer for site directed mutagenesis |
| Sequence-based reagent | Ctgcagacccagtgttgc ggtatcaacggcaac |
Life Technologies | NrnCBh
E27A_b |
Primer for site directed mutagenesis |
| Sequence-based reagent | Cgatgcggctgcgcac ccagtgtttcggtatcaacg |
Life Technologies | NrnCBh
L31A_a |
Primer for site directed mutagenesis |
| Sequence-based reagent | Cgttgataccgaaaca ctgggtgcgcagc cgcatcg |
Life Technologies | NrnCBh
L31A_b |
Primer for site directed mutagenesis |
| Sequence-based reagent | Agatcgaaacgacca aaggcaaagattttg gtaatatcacgatcgc |
Life Technologies | NrnCBh
H79A_a |
Primer for site directed mutagenesis |
| Sequence-based reagent | Gcgatcgtgatattacc aaaatctttgcctttg gtcgtttcgatct |
Life Technologies | NrnCBh
H79A_b |
Primer for site directed mutagenesis |
| Sequence-based reagent | Gtgccagaattgccag agcgaaacgac caaagtga |
Life Technologies | NrnCBh
D84A_a |
Primer for site directed mutagenesis |
| Sequence-based reagent | Tcactttggtcgtttcgct ctggcaattctggcac |
Life Technologies | NrnCBh
D84A_b |
Primer for site directed mutagenesis |
| Sequence-based reagent | Cgggtcagtttgcttgc aattgcggtacaa aaaacaacatccgg |
Life Technologies | NrnCBh
K103A_a |
Primer for site directed mutagenesis |
| Sequence-based reagent | Ccggatgttgttttttgtacc gcaattgcaagca aactgacccg |
Life Technologies | NrnCBh
K103A_b |
Primer for site directed mutagenesis |
| Sequence-based reagent | Acatcacttgctgcagct tcaatctgtgcac ggctcagg |
Life Technologies | NrnCBh
Y151A_a |
Primer for site directed mutagenesis |
| Sequence-based reagent | Cctgagccgtgcacag attgaagctgca gcaagtgatgt |
Life Technologies | NrnCBh
Y151A_b |
Primer for site directed mutagenesis |
| Sequence-based reagent | Cggtgcagatacaga acagcacttgctgcatattcaa | Life Technologies | NrnCBh
D155A_a |
Primer for site directed mutagenesis |
| Sequence-based reagent | Ttgaatatgcagcaag tgctgttctgtatctgcaccg |
Life Technologies | NrnCBh
D155A_b |
Primer for site directed mutagenesis |
| Sequence-based reagent | Gctcaagcttgaattc gctggctgcaa agatatcaatttcgc |
Life Technologies | NrnCBh
H205A_a_pJHA |
Primer for site directed mutagenesis |
| Sequence-based reagent | Gcgaaattgatatctttgc agccagcgaattcaagcttgagc | Life Technologies | NrnCBh
H205A_b_pJHA |
Primer for site directed mutagenesis |
| Sequence-based reagent | Gtgcggccgcttagctgg ctgcaaagatatcaatttcgct | Life Technologies | NrnCBh
H205A_a_SUMO |
Primer for site directed mutagenesis |
| Sequence-based reagent | Agcgaaattgatatcttt gcagccagctaa gcggccgcac |
Life Technologies | NrnCBh
H205A_b_SUMO |
Primer for site directed mutagenesis |
| Sequence-based reagent | 5′-GG-3′ (RNA primer) | Sigma | ||
| Sequence-based reagent | 5′-AGG-3′ (RNA primer) | Sigma | ||
| Sequence-based reagent | 5′-AAGG-3′ (RNA primer) | Sigma | ||
| Sequence-based reagent | 5′-AAAGG-3′ (RNA primer) |
Sigma | ||
| Sequence-based reagent | 5′-AAAAGG-3′ (RNA primer) |
Sigma | ||
| Sequence-based reagent | 5′-AAAAAGG-3′ (RNA primer) |
Sigma | ||
| Sequence-based reagent | 5′-pGG-3′ (RNA primer) | Biolog’ catalog number P023-01 | ||
| Sequence-based reagent | 5′-pAA-3′ (RNA primer) | Biolog’ catalog number P033-01 | ||
| Sequence-based reagent | 5′-pGC-3′ (RNA primer) | GE Healthcare Dharmacon | ||
| Chemical compound, drug | 5′-pAp-3′ (RNA primer) | Sigma | Cat# A5763 | |
| Sequence-based reagent | Various RNA and DNA oligonucleotides |
IDT | ||
| Antibody | Anti-HA (rabbit polyclonal) |
Takara | Cat# 631207 | (1:100) |
| Antibody | Anti-HA−agarose (mouse monoclonal, clone HA-7) |
Sigma | Cat# A2095; RRID:AB_257974 | (10 µl) |
| Antibody | Anti-rabbit (donkey polyclonal, HRP-conjugated) |
Cytiva | Cat# NA934 | (1:5000) |
| Software, algorithm | Prism | GraphPad | RRID:SCR_002798 | |
| Software, algorithm | XDS Program Package | Kabsch, 2010; PMID:20124693 | RRID:SCR_015652 | Distributed through SBGrid |
| Software, algorithm | Pointless | Evans, 2006; PMID:16369096 | RRID:SCR_014218 | Distributed through SBGrid |
| Software, algorithm | Scala | Evans, 2006; PMID:16369096 | Distributed through SBGrid | |
| Software, algorithm | Phenix | Adams et al., 2010; PMID:20124702 | RRID:SCR_014224 | Distributed through SBGrid |
| Software, algorithm | Coot | Emsley et al., 2010; PMID:20383002 | RRID:SCR_014222 | Distributed through SBGrid |
| Software, algorithm | MrBump | Keegan et al., 2018;PMID:29533225 | Distributed through SBGrid | |
| Software, algorithm | PyMOL | Schrödinger | RRID:SCR_000305 | Distributed through SBGrid |
| Software, algorithm | UCSF ChimeraX | Pettersen et al., 2021; PMID:32881101 | RRID:SCR_015872 | Distributed through SBGrid |
| Software, algorithm | cryoSPARC | Punjani et al., 2017; PMID:28165473 | RRID:SCR_016501 | |
| Software, algorithm | RELION | Zivanov et al., 2018; PMID:30412051 | RRID:SCR_016274 | |
| Software, algorithm | GCTF | Zhang, 2016; PMID:26592709 | RRID:SCR_016500 | |
| Software, algorithm | iTOL | Letunic and Bork, 2019; PMID:30931475 | RRID:SCR_018174 | |
| Software, algorithm | TCoffee | Notredame et al., 2000; PMID:10964570 | RRID:SCR_019024 | |
| Software, algorithm | Hmmer | Eddy, 2011; PMID:22039361 | RRID:SCR_005305 | |
| Software, algorithm | MAFFT | Katoh et al., 2005; PMID:15661851 | RRID:SCR_011811 | |
| Software, algorithm | SnakeMake | Köster and Rahmann, 2018; PMID:29788404 | RRID:SCR_003475 |