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Isolation of the PKH3 gene. Transformants of the yeast strain INA106-3B (pkh1D398G pkh2Δ::LEU2) carrying the indicated plasmids were streaked onto YPGlu medium and incubated at 35°C. Each patch represents an independent transformant. Plasmids were YEp13 (vector), YEp-PKH3 (PKH3), and YCplac111-PKH1 (PKH1).
