Figure 2.

POW NS5 gene sequence recovered from the infecting tick sample demonstrating identity with deer tick virus sequences. A 290bp portion of the NS5 gene, amplified with primers POW1/POW2 [2], was sequenced and aligned with related DTV and POW sequences downloaded from GenBank (accession numbers on tree). A neighbor-joining consensus tree was constructed using Tamura-Nei algorithm for genetic distances with 500-bootstrap replicates using Geneious v10 (Biomatters). Central European encephalitis virus was used as the outgroup. Abbreviations: DTV, deer tick virus; POW, Powassan.