Table 1.

Gradient elution parameter.

Time (min)	A phase: 0.1% FA/water	B phase: 0.1% FA/ACN	Flow rate (nl/min)
0	95%	5%	600
16	90%	10%	600
51	78%	22%	600
71	70%	30%	600
72	5%	95%	600
78	5%	95%	600