Figure 5.

ITPR1 reverses the regulatory effect of LINC00662 knockdown in OS cells. (a) U2OS cells were transfected with sh-NC, sh-LINC00662, or sh-LINC00662 + pcDNA3.1/ITPR1, and the expression of ITPR1 in the transfected cells was measured by RT-qPCR. (b-c) Cell viability and proliferation in the transfected U2OS cells were detected using MTT and colony formation assays. (d-e) Cell invasion and migration in the transfected U2OS cells were evaluated by transwell invasion and migration assays. (f-g) Stem cell properties in the transfected U2OS cells were detected by western blot and sphere formation assays. ^∗∗P < 0.01.