Skip to main content
. 2021 Sep 8;6(17):e150249. doi: 10.1172/jci.insight.150249

Figure 3. miR-182-5p promotes thermogenic gene expression in adipocytes via a macrophage-dependent mechanism.

Figure 3

(A) qRT-PCR analysis of mRNA levels of M1 and M2 marker genes in sWAT of miR-182-5p agomir- or control agomir-injected (fat pad) mice (n = 4–6/group). (B) Flow cytometry analysis showing the percentage of total stromal vascular fractions (SVFs), and M1 and M2 macrophages in sWAT of miR-182-5p agomir- or control agomir-injected (fat pad) mice (n = 4–5/group). qRT-PCR analysis for mRNA levels of M1 and M2 marker genes (C) and flow cytometry analysis showing the percentage of M1 and M2 macrophages in SVFs (D) from sWAT of miR-182-5p+/– and WT control mice under cold exposure conditions (n = 3–4/group). miR-182-5p mimic (182-mimic) or its negative control (nc-mimic) were overexpressed in mouse primary adipocytes. The cells were cultured alone or cocultured with mouse peritoneal macrophages (PM) for 3 days (n = 3–4/group). (E) The Ucp1 mRNA levels were determined in mouse primary adipocytes by qRT-PCR. (F) qRT-PCR analysis for mRNA levels of M1 and M2 marker genes. Data represent mean ± SEM. Significance determined by unpaired 2-tailed Student’s t test (A–D and F) and by 1-way ANOVA (E). *P < 0.05; **P < 0.01.