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. 2021 Sep 22;6(18):e149301. doi: 10.1172/jci.insight.149301

Figure 4. miR-214-3p is upregulated in Nox2–/– Tregs and controls CCR4 and CCR8 mRNA expression.

Figure 4

(A) Expression of miR-214-3p in Nox2–/– or WT Tregs (n = 6). (B and C) CCR4 and CCR8 mRNA expression in Jurkat T cells transfected with miR-214-3p mimetic or inhibitor. Some cells were treated with the Nox2 inhibitor gp91ds-tat (30 μM) for 24 hours. Control cells were transfected with miR-negative control and incubated with sc-tat for 24 hours. Graphs represent 2 independent experiments. (D–F) Levels of miR-214-3p and mRNA coding for CCR4 and CCR8 in Jurkat T cells incubated 24 hours with PEG-SOD (20 IU/ml) and PEG-catalase (300 IU/ml). Graphs represent 3 independent experiments. (G–I) Reporter assay using CCR4, CCR4 untranslated region, and CCR8 constructs. Graphs represent 2 independent experiments. Data are shown as mean ± SEM. *P < 0.05 for indicated comparisons, Mann-Whitney 2-tailed t test in A, D–F, and H and Kruskal-Wallis followed by Dunn’s post test in B, C, G, and I.