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. 2021 Sep 8;6(17):e140173. doi: 10.1172/jci.insight.140173

Figure 6. CrT deficiency causes greater infarct and ATP depletion after cerebral ischemia.

Figure 6

(A) LC-MS quantification of the brain ATP, Cr, and PCr levels in contralateral (Con) and ipsilateral (Ipsi) hemispheres in CrT+/y versus CrT–/y mice 24 hours after photoactivation (n = 4 for each). (B and C) Immunoblotting and quantification of the p-AMPK/autophagy and mTOR signaling pathway activity in P16 CrT+/y versus CrT–/y mice 24 hours after photoactivation directed at the proximal branch of the middle cerebral artery. The protein expression levels in the contralateral hemisphere of CrT+/y mice were used as the baseline. (D and E) Representative brain sections and quantification of the infarct size in untreated CrT+/y (n = 10) and CrT–/y mice (n = 14), intranasal 184 mg/kg Cr–treated CrT+/y (n = 12) and CrT–/y mice (n = 11), and intraperitoneal 200 mg/kg Cr–treated CrT+/y (n = 7) and CrT–/y mice at 16 days of age (n = 9). The intranasal and intraperitoneal Cr treatment was administered within 30 minutes after photoactivation. The violin plots in C are representative of 3 independent experiments, with n = 3 biological replicates; all other data are shown as mean ± SEM. All statistical analyses were performed using 2-way ANOVA followed by Tukey’s multiple comparison post hoc test. IN, intranasal; IP, intraperitoneal.