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. 2021 Sep 22;6(18):e150405. doi: 10.1172/jci.insight.150405

Figure 3. Selective loss of miR-150 in cardiomyocytes induces cardiac apoptosis post-MI.

Figure 3

(A and B) Representative TUNEL staining images in heart sections of periischemic border area at 4 weeks after MI (A) and quantification of apoptosis in six 40× fields (B). Scale bars: 100 μm. (C and D) Representative cleaved caspase 3 staining images in heart sections of periischemic border area at 4 weeks after MI (C) and quantification of apoptosis in six 40× fields (D). Scale bars: 100 μm. (E) qPCR analysis of proapoptotic p53 expression in miR-150 cKO hearts compared with miR-150fl/fl controls at 4 weeks after MI. Data are shown as fold induction of gene expression normalized to Gapdh. n = 6 per group. One-way ANOVA with Tukey multiple-comparison test. *P < 0.05, **P < 0.01, or ***P < 0.001 versus sham; #P < 0.05 or ##P < 0.01 versus MI miR-150fl/fl. Data are presented as mean ± SEM.