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. 2021 Sep 22;6(18):e150405. doi: 10.1172/jci.insight.150405

Figure 8. Sprr1a is necessary for miR-150–dependent regulation of cardiomyocyte apoptosis.

Figure 8

(A and B) H9c2 cells were transfected with control scramble siRNA (si-control) or Sprr1a siRNA (si-Sprr1a) (A) and with antimiR control scramble or antimiR-150 (B). qPCR for Sprr1a (A) or miR-150 (B) were performed to check the knockdown efficiency. n = 6 per group. Unpaired 2-tailed t test. ***P < 0.001 versus si-control or anti-miR control. (C–E) RNA interference with Sprr1a protects CMs from the proapoptotic effects of anti–miR-150. CMs were subjected to in vitro simulation of I/R (sI/R). TUNEL assays were then performed in both normoxic (C and E) and sI/R conditions (D and E). The percentage of apoptotic nuclei (green) was calculated by normalizing total nuclei (blue). The quantification of data is from 6 independently obtained biological samples. One-way ANOVA with Tukey multiple-comparison test. *P < 0.05 or **P < 0.01 versus control: either si-control or antimiR control. ##P < 0.01 versus antimiR-150. Data are presented as mean ± SEM.