TABLE 1.

Repair of a genomic DSB in the absence or presence of a homologous donor

Donor and cell linea	Electroporationb		Total no. of HAT-resistant colonies	Frequency of HAT-resistant colonies (10−5)c
	Construct used	No.
No donor
Sce-3	I-SceI	42	157	0.03
	pCMV-I-SceI	10	650	0.65
	Mock	13	0	0
360-bp donor
360-1	pCMV-I-SceI	20	2,600	2.6
	Mock	5	0	0
360-2	pCMV-I-SceI	20	2,000	2.0
	Mock	5	0	0
2-kb donor
26-1	pCMV-I-SceI	5	673	2.7
	Mock	5	101	0.4
26-2	pCMV-I-SceI	8	1,156	2.9
	Mock	8	58	0.14

^aCell line Sce-3 contains an integrated copy of pTKSce (Fig. 1B), cell lines 360-1 and 360-2 each contain an integrated copy of pTKSce-360 (Fig. 1B), and cell lines 26-1 and 26-2 each contain an integrated copy of pTKSce-26 (Fig. 1B). 

^bCell line Sce-3 was electroporated with 150 U of I-SceI endonuclease or 5 μg of pCMV-I-SceI per electroporation. Other cell lines were electroporated with 10 μg of pCMV-I-SceI per electroporation. Mock electroporations were with PBS alone. 

^cTotal number of HAT-resistant colonies recovered divided by total number of cells electroporated.