TABLE 2.
Summary of Southern blotting analysis of HAT-resistant segregants from cell lines 26-1 and 26-2
| Event type and cell line | No. of HAT-resistant segregants analyzed | No. of HAT-resistant segregants displaying:
|
||
|---|---|---|---|---|
| HR | NHEJ | Amplificationa | ||
| DSB-induced | ||||
| 26-1 | 12 | 7b | 5 | 0 |
| 26-2 | 30 | 5c | 12 | 13 |
| Spontaneous | ||||
| 26-2 | 20 | 20d | 0 | 0 |
Thirteen HAT-resistant segregants from cell line 26-2 displayed an amplification of sequences in the vicinity of the I-SceI-induced DSB, in conjunction with NHEJ. These 13 clones are distinct from the 12 segregants from cell line 26-2 listed under NHEJ. See text for details.
Among the recombinants, six were gene conversions of the I-SceI recognition sequence insertion and one was a crossover.
Among the recombinants, all five were gene conversions of the I-SceI recognition sequence insertion.
Among the recombinants, 18 were gene conversions of the I-SceI recognition sequence insertion, 1 was a gene conversion of the XhoI linker insertion, and 1 was a crossover.