TABLE 3.
Precise religation as a mode of intrachromosomal DSB repair
| Cell line | No. of electroporations | Frequency of HAT-resistant colonies (10−4)a | No. of HAT-resistant colonies analyzed | XbaI site loss (%)b | Precise religation (%)c |
|---|---|---|---|---|---|
| Sce2-1 | 3 | 2.2 | 39 | 44 | 36 |
| Sce2-2 | 1 | 2.4 | 17 | 65 | 65 |
| Sce2-3 | 1 | 0.068 | 15 | 33 | 27 |
| Sce2-4 | 1 | 0.032 | 6 | 50 | 33 |
a
Number of HAT-resistant colonies divided by total number of cells electroporated.
b
Percentage of analyzed HAT-resistant clones that lost the XbaI site between the I-SceI sites (Fig. 5), based on PCR analysis.
c
Percentage of analyzed HAT-resistant clones that had undergone precise religation, based on nucleotide sequence determination.