Extended Data Fig. 2. TEs bind to MDA5 upon stress in human and mouse cells.
a, Bar graphs depicting the mean counts of LINE, SINE, LTR and DNA transposon (DNA) RNA (fold change >1.5 and p-value < 0.05) bound to MDA5 or GFP after irradiation or decitabine treatment (sense DNA strand-upper panel, antisense DNA strand-lower panel) (n = 2 biologically independent population samples, 2 independent experiments). b, Bar graphs depicting the mean counts of LINE, SINE, LTR and DNA transposon (DNA) RNA (fold change >1.5 and p-value < 0.05) bound to MDA5 after irradiation or decitabine treatment or to MDA5 without treatment (sense DNA strand-upper panel, antisense DNA strand-lower panel) (n = 2 biologically independent population samples, 2 independent experiments). c, Representative track that shows binding of L1M4c to GFP after irradiation or decitabine treatment and to MDA5 without treatment or after irradiation or decitabine treatment. Y-axis represents RPKM. Crosslinking events are also shown. d-e qPCR experiments after FLASH depicting binding of TEs to GFP or MDA5 after irradiation or decitabine treatment (d) or to MDA5 without treatment or after irradiation or decitabine treatment (e) (n = 2 biologically independent samples and experiments). f, qPCR experiment after FLASH from mouse OP9 cells depicting binding of LINE1 elements to GFP or MDA5 without treatment or after irradiation (n = 2 biologically independent samples and experiments).