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. 2021 Jul 12;23(7):704–717. doi: 10.1038/s41556-021-00707-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a, Relative changes in median fluorescence intensity (MFI) of phosphorylated IRF3 (pIRF3) in WT or Mda5^−/− HSCs at D0, H16 or D3 after 5-FU treatment, normalized to the WT D0. n = 8 biologically independent samples in n = 3 independent experiments (D0), n = 10 biologically independent samples in n = 3 independent experiments (H16) and n = 4 biologically independent samples in one experiment (D3). Each dot represents one mouse. Data are mean ± s.d. Statistical analysis was performed using two-tailed t-tests; n.s. not significant. b, The amount of IFNβ (pg ml⁻¹) measured in the BM serum of WT or Mda5^−/− mice at D0, H16 or D3 after 5-FU treatment. Each dot represents one mouse. n = 14 (D0), n = 6 (H16), n = 10 (D3) biologically independent samples in n = 2 (D0 and D3) and n = 1 (H16) independent experiments. Data are mean ± s.d. Statistical analysis was performed using two-tailed t-tests; n.s., not significant. c, The integrated density of the NF-κB subunit p65 in the cytoplasm and the nucleus of WT or Mda5^−/− HSCs at H16 after 5-FU treatment. n = 129 (WT) and n = 132 (Mda5^−/−) HSCs examined in n = 2 independent experiments. Statistical analysis was performed using two-tailed t-tests; n.s., not significant. d, Immunostaining for NF-κB subunit p65 in WT or Mda5^−/− HSCs at D0 and H16 after 5-FU treatment. n = 2 independent experiments. Scale bar, 5 μm. The histograms on the right represent the grey value intensity of both p65 (green) and Hoechst (blue) as indicated in the figure by the red dashed line.

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