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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: Mol Cancer Res. 2021 May 26;19(10):1739–1750. doi: 10.1158/1541-7786.MCR-21-0005

Figure 1. Representative confocal microscopic images of transverse tibial bone marrow sections containing human breast cancer cells that were inoculated into animals pre-treated with 223Ra dichloride and assayed for γ-H2AX formation.

Figure 1.

MDA-MB-231 (A–C) and MCF-7 (D–F) labeled with CellTracker™ Green CMFDA and stained with anti-γ-H2AX antibody (AlexaFluor™ 568 red) to visualize DNA damage. The tumor, bone marrow, and cortical bone have all been demarcated (A). Nuclear counter-staining with DAPI (blue) visualizes DNA damage in mouse osteocytes in the tibiae of animals inoculated with MDA-MB-231 cells (G–I) and MCF-7 cells (J–L). The inner (endosteum) and outer (periosteum) cell layers surrounding the cortical bone have been noted (G). Arrowheads delineate γ-H2AX positive cells. Images acquired with a Nikon A1R microscope with CFI Apochromat TIRF 60XC oil (NA 1.40), DS-Fi3 camera and NIS-Elements C software.