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. Author manuscript; available in PMC: 2022 Oct 15.
Published in final edited form as: J Immunol. 2021 Sep 22;207(8):2077–2085. doi: 10.4049/jimmunol.2100404

Figure 1: Enrichment of differentiated phenotype in CMV-IE-1 responsive cells:

Figure 1:

A) PBMC were stimulated with IE-1 peptide for 5 hours, stained for IFNγ capture and index sorted. Gates were on live singlet lymphocytes, CD3+ and CD14−CD16−CD19−, and as shown. TCR and other genes were amplified and sequenced. Protein expression data analyzed to identify the frequency of aged (CD57+, B and C) and TEMRA (CD45RA+CD27−, D and E) in total and sorted CD8 (B and D) and CD4 (C and E) T cells. Graphs include individual data points and box plot showing median and interquartile range. Statistics computed with two-way ANOVA with Sidak’s correction for multiple comparisons. *** represents p<0.001, **** represents p<0.0001. n=6 subjects.