Fig. 3. The effect of cytokinin on the juvenile-to-adult phase transition is not dependent on miR156.

a miR156 abundance in whole shoots of genotypes with an altered CK status compared to the wild type (n = 4 biological replicates). Dots indicate each single biological replicate. Asterisks indicate significant differences compared to the wild type of the respective time point, as calculated by one-way ANOVA, post-hoc Dunnett’s test (*p < 0.05; **p < 0.01; ***p < 0.001). See also Supplementary Fig. 2a. b miR156 expression kinetics in 10-days-old SD-grown wild-type seedlings after treatment with 1 µM BA (n = 6 biological replicates). Statistical analysis was performed using one-way ANOVA, post-hoc Dunnett’s test. No statistically significant differences were observed for miR156, comparing each time point with time point 0 (p < 0.05). Transcript levels (a, b) were determined by qRT-PCR. Data were normalized to TAFII15. Data displayed are expressed as mean ± SEM. c Number of leaves without abaxial trichomes in ahk2 ahk3 MIM156 hybrid plants grown in SD. In the box plot, the center line represents the median value and the bounderies indicate the 25th percentile (upper) and the 75th percentile (lower). The X marks the mean value. Whiskers extend to the largest and smallest value, excluding outliers which are shown as dots. Letters indicate significant differences between the genotypes, as calculated by Kruskal–Wallis test (p < 0.05). Exact p-values calculated for a–c are included in the Source Data files.