Fig. 2.

Effects of SARS-CoV-2 Spike protein on miR-93-5p content and NF-κB p105/p50 production. (A) IB3-1 cells were exposed for 24 h to S-protein. RNA was isolated and miR-93-5p content quantified by RT-qPCR. (B,C) Proteins were purified from IB3-1 cells treated as described in (A), and Western blotting performed (see the Methods section) using antibodies recognizing NF-κB p105/p50 and β-actin. (B) Representative results indicating S-protein mediated upregulation of NF-κB; (C) quantitative analysis. The relative expression (average values ± SD) of NF-κB p105/p50 is indicated (n = 3).