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. 2021 Sep 21;53(9):1379–1389. doi: 10.1038/s12276-021-00671-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a, t-SNE plots of the SMC subpopulations (SMC_1, SMC_2, SMC_3, SMC_4, and SMC_5) from the ND (2,601 cells) and HFD (2,370 cells) groups. b, Percentages of the SMC subpopulations from the ND (SMC_1, 43%; SMC_2, 37%; SMC_3, 14%; SMC_4, 4%; and SMC_5, 1%) and HFD (SMC_1, 64%; SMC_2, 23%; SMC_3, 12%; SMC_4, 0.8%; and SMC_5, 0.2%) groups. c, Heatmap of the top 20 marker genes per subpopulation. d–f, Top 10 pathways associated with the SMC_1 (d), SMC_2 (e), and SMC_3 (f) clusters. g, Feature plots of the expression of selected marker genes for the SMC subpopulations from the ND and HFD groups. h, Violin plots of the expression of selected marker genes for the SMC subpopulations from the ND and HFD groups. i, Expression of extracellular matrix (ECM) degradation-associated genes for SMC subpopulations as shown by violin plots.