Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Oct 5;11:19732. doi: 10.1038/s41598-021-98661-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Laser disruption of polyQ::YFP aggregates, in 4 and 8 day-old AM141 nematodes. (a) Survivals for lifespan (*Cox–Mantel log-rank p ≈ 0.003, day 8 laser vs. control). (b,c) Video analyses of worm-motility data (travel speed, flexion frequency). Each day-8 laser group differed from its control (b, p< 6E–5; c, p < 0.01). Dashed lines in (b) show linear trend analysis for travel speed. Inset (right of c) shows a video frame with two worm positions connected by a midpoint trace. (d) Aggregate count (yellow) and total fluorescence (green) per worm. Each day-1/after differed from day-1/before (t test p < 0.05). Each day-2 and day-3 post-treatment group differed from day-1/after (p < 0.005). (e) Fluorescence images of laser-treated worms before, immediately after, and 48 h after laser therapy (dashed circles highlight two aggregates with post-therapy recovery of fluorescence). (f) Release of non-aggregate (diffuse) fluorescence. Error bars show SDs, unless omitted for clarity. Treatment differs from control at days 10–11 (combined p < 3E–5).