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. 2021 Oct 5;6:119. doi: 10.1038/s41541-021-00382-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a An outline of the treatment schedule. CMS5-VP1 tumor cells at 1 × 10⁶ per mouse were subcutaneously inoculated into the left flank back of BALB/c mice on day 0. b When tumors were palpable on day 5, tumor-bearing mice were randomly divided and immunized with VP1/CRA, VP1/A, CRA, and PBS thrice at 1-week intervals. c To evaluated the durable antitumor effects induced by triple treatment of VP1/CRA, tumor-free mice were rechallenged with CMS5-VP1 tumor cells at 1 × 10⁶ per mouse in the right flank back on day 49. Tumors were measured with digital calipers and tumor volumes were calculated.