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. 2021 Oct 5;12:5801. doi: 10.1038/s41467-021-25821-y

Fig. 3. Yolk venting is promoted by insulin/IGF-1 signalling (IIS).

Fig. 3

a Yolk venting is downregulated by reduced IIS and upregulated by enhanced IIS. Quantitated YP170 band on protein gels. Means ± S.E.M. of 3 trials (n = 100 worms per trial). One-way ANOVA (Dunnett’s correction) or unpaired two-tailed t-test. Left to right for stars P = <0.0001, <0.0001, 0.049, 0.561 (ns), 0.046. b Decreased yolk milk provisioning by reduced IIS reduces larval growth on preconditioned plates relative to wild-type, and vice versa for increased IIS. Combined data of 3 trials (n = 200 worms per trial). Left to right for stars P = 0.019, 0.114 (ns), <0.0001, <0.0001, <0.0001. c Decreased larval growth on plates with daf-2(e1370) surrogate mothers. Wild-type mothers were allowed to lay their last eggs and either left in situ or replaced. P = <0.0001. b, c Tukey box plots of length measurement of L1 larvae (line at median; + at mean; box limits are 25th and 75th percentiles; whiskers denote 1.5 times the interquartile range). Non-parametric Kolmogorov–Smirnov two-tailed tests performed and corrected according to FDR. d Unfertilised oocyte production is downregulated by reduced IIS and upregulated by increased IIS. Bottom: Number laid for 24 h on d4–6 of adulthood and normalised to wild type. Means ± S.E.M. of 3 trials (n = 10 worms per trial). One-way ANOVA (Dunnett’s correction) or unpaired two-tailed t-test. Left to right for stars P = 0.005, 0.033, 0.002, <0.0001, <0.0001. Top: NGM plates imaged after 15 wild-type, daf-18(nr2037) or daf-2(e1370) worms were left for 24 h. Black arrowheads: unfertilised oocytes; white arrowhead: yolk pool. Red: treatments that increase the dependant variable; blue: that reduce the dependant variable; white: no effect; and grey: control. Scale 100 μm. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.