Table 1.

The sequences of primers used for real-time qPCR.

Genes	Sequence
si-MRTF-A	5′-GGACGACCTGTTTGACATT-3′
si-SRF	5′-CCCUGUUUCAGCAGUUCAGTT-3′
si-p65	5′- CAAG ATCAATGGCTACACA-3′
si-β-catenin	5′- GCTTGGAA TGAGACTGCTG-3′
si-Smad2	5′- GAAUUGAGCCACAG AGUAAUU-3′
si-Smad3	5′-GAGUUCACUCCACAUUCUCUU-3′
β-actin	F: 5′- CGAGATCCCTCCAAAATCAA-3′
	R: 5′-TTCACACCCATGACGAACAT-3′
MRTF-A	F: 5′-ACCGTG ACCAATAAGAATGC-3′
	R: 5′-CCGCTCTGAATGAGAATGTC-3′
SRF	F: 5′-TGCCTTCAGTAGGAACAAGC-3′
	R: 5′-ACTTCCATCTTG GCACCC-3′
PD-L1	F: 5′-AGACCACCACCACCAATTCC-3′
	R: 5′-TGGAGGATGTGCCAGAGGTA-3′
PD-L1 -Mut- luc	F: 5′-CGACGCGTGAGACA CTCTGAGAAACAGCAC-3′
	R: 5′-CCGCTCGAGCTCTGCCCAAGGCA GCAAATC-3′
Fragment I	F: 5′-CTCCATGCTCCTGCCAA-3′
	R: 5′-ACCTCAAGTGATCCGCC-3′
Fragment II	F: 5′-AAAAGGGAGCACACAGG-3′
	R: 5′-AAAAAGTCAGCAGCAGA-3′
Fragment III	F: 5′-ATATCAAGTTATGTCAA-3′
	R: 5′-GTCCCGCCCACCTCTGC-3′
Fragment IV	F: 5′-TGAGGTCAAGGAGTT-3′
	R: 5′-TGGGTTAGTGAATGG-3′
Fragment V	F: 5′-GGCTTTCTTAACCCTCAC-3′
	R: 5′-AACTTCATTTGCTTTGTC-3′
Fragment VI	F: 5′-GTCACAGAATCCACG-3′
	R: 5′-TAAAAAGTCAGCAGC-3′
SgRNA-MRTF-A	F: 5′-CACCGCTGACCAGCTCCGATCTCTC-3′
	R: 5′-AAACGAG AGATCGGAGCTGGTCAGC-3′