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. Author manuscript; available in PMC: 2021 Dec 11.
Published in final edited form as: Pediatr Res. 2021 Apr 6;90(5):1086–1092. doi: 10.1038/s41390-021-01484-9

Table 2.

Details of blood cultures and clinician-reported documentation

Total
n = 987
Birth weight
>1500 gramsa
n = 720
VLBW & culture obtained ≤7 days
n = 107
VLBW & culture obtained >7 days
n = 160
p-value
Clinician-reported total volume per order, n (%)b
 2 mL documented and 2 bottles sent 807 (81.8) 617 (85.7) 86 (80.4) 104 (65.0) <0.001
 1 mL documented and 1 bottle sent 124 (12.6) 66 (9.2) 15 (14.0) 43 (26.9) <0.001
 Documented <1 mL per bottle sent 30 (3.0) 18 (2.5) 1 (0.9) 11 (6.9) 0.006
 Missing documentation 26 (2.6) 19 (2.6) 5 (4.7) 2 (1.3) 0.24
Site for specimen draw, n (%)
 Phlebotomy 787 (79.7) 664 (92.2) 18 (16.8) 105 (65.6) <0.001
 Peripheral arterial line 8 (0.8) 2 (0.3) 1 (0.9) 5 (3.1)
 Umbilical line 145 (14.7) 53 (7.4) 85 (79.4) 7 (4.4)
 Peripherally inserted central catheter 47 (4.8) 1 (0.1) 3 (2.8) 43 (26.9)
Bottles sent, n (%)
 Aerobic-Anaerobic pair 822 (83.3) 625 (86.8) 88 (82.2) 109 (68.1) <0.001
 Aerobic/Anaerobic alone 135 (13.7) 70 (9.7) 16 (15.0) 49 (30.6)
 Missing documentation 30 (3.0) 25 (3.5) 3 (2.8) 2 (1.3)
More than 2 attempts at phlebotomy, n (%) 98/787 (12.5) 83/664 (12.5) 4/18 (22.2) 11/105 (10.5) 0.38
 Missing documentation 20/787 (2.5) 20/664 (3.0) 0 0
Orders growing any organism, n (%) 61 (6.2) 10 (1.4) 3 (2.8) 48 (30.0) <0.001
 Contaminants 7/61 (11.5) 5/10 (50.0) 1/3 (33.3) 1/48 (2.1) <0.001
 CoNS (not treated as contaminant) 10/54 (18.5) 1/5 (9.3) 1/2 (50.0) 8/47 (17.0) 0.13

Abbreviations: VLBW – very low birth weight, CoNS – Coagulase negative staphylococci

a

All blood culture orders for infants with birth weight >1500 grams were drawn at ≤7 days after birth except 10 orders (among 9 infants) drawn >7 days.

b

Assessment of inoculate volume adequacy based on documented note, e.g. 2 mL and 2 bottles sent, the assumption was that each bottle contained ≥1 mL; if 1.8 mL sent in 2 bottles, then categorized as <1 mL per bottle.