Fig. 2.

Late-life EOD fasting affects body weight-adjusted resting metabolism. a, b Average food intake (kcal/g of body weight) per mouse over the 4-day period in the metabolic chamber was determined by manual weighing daily in the male (a) and female (b) Chow AL and Chow EOD groups (n = 3–6 mice per sex per group). c–h The body weight-adjusted average vO₂ (mL/kg/hr) (c, d), vCO₂ (mL/kg/hr) (e, f), and heat (kcal/kg/hr) (g, h) were measured approximately every 20 min. The figures (c–h) depict the mean with no error bars for a representation purpose, with insets providing the area under the curve (AUC) of combined/summation of area, fast day 12:12 light:dark cycle, and fed day 12:12 light:dark cycle for each measure. The inset figures depict the mean with error bars (± SEM). The asterisks indicate the significant difference between the same-sex Chow AL and Chow EOD groups. *p < 0.05, **p < 0.01, and ****p < 0.0001. See also Supplemental Figure 2